Microfluidic 3D cultures
In bone external mechanical load is mediated by interstitial fluid flow, which represents the messenger between the bone cells and the biomechanical cues that are induced by our physical activity. These cues contribute to the biomechanical bone adaption response. In vitro studies often use 2D flat substrates that provide important information on bone cell response to biomechanical stimuli. However, this approach may be limited, compared to the in vivo situation because the 3D organization of bone cell morphology, the matrix attachment and the pericellular fluid flow environment in vivo differ significantly from 2D monolayer cultures. By using chip-based 3D cultures of bone cells in microfluidic bioreactors and compressive force devices, we will focus on how bone cell functions are modulated by their biomechanical microenvironment in the context of bone regeneration.
Altmann B, Löchner A, Swain M, Kohal R-J, Giselbrecht S, Gottwald E, Steinberg T, Tomakidi P. (2014). Differences in morphogenesis of 3D cultured primary human osteoblasts under static -and microfluidic growth conditions. Biomaterials; 35, 3208 – 19
Gottwald E, Kleintschek T, Giselbrecht S, Truckenmüller R, Altmann B, Worgull, Döpfert M, Schadg L, Heilman M. Characterization of a chip-based bioreactor for three-dimensional cell cultivation via magnetic resonance imaging. Journal of Medical Physics, 2013, 23 (2), 102-10
Altmann B, Giselbrecht S, Rieke M, Welle A, Weibezahn K F, Gottwald E. (2010). Chip-Based Tissue Engineering in Microbioreactors. In: Methods in Bioengineering: 3D Tissue Engineering. Berthiaume F and Morgan J editors. Boston: Artech House, pp. 83-99
Doctor of Philosophy (Biology), Heidelberg University and Institute for Biological Interfaces-1, Karlsruhe Institute of Technology, Germany
Department of Prosthodontic Dentistry, Center for Dental Medicine, University of Freiburg, Germany
Institute for Biological Interfaces-1, Karlsruhe Institute of Technology, Germany