OSTEOGENESIS ON A CHIP 2018-03-05T12:49:18+00:00
Osteogenesis on a chip

Innovative three-dimensional (3D) cell culture techniques are becoming increasingly important in hard tissue research, as a growing number of in vitro studies point to more tissue-specific bone cell behavior in response to physiologically orientated 3D microenvironments, compared to traditional 2D-monolayer configurations. In this context, we are using a micro-structured polymer chip as a scaffold, which enables cells to self-assemble into adherent multicellular aggregates of uniform size. Due to the specialized characteristics of the chip design, bone cells can be cultured successfully under 3D static conditions but also under dynamic conditions when using a compatible microfluidic bioreactor.

Publications

Altmann B, Steinberg T, Giselbrecht S, Gottwald E, Tomakidi T, Bächle-Haas M, Kohal R-J. (2011). Promotion of osteoblast differentiation in 3D biomaterial micro-chip arrays comprising fibronectin-coated poly(methyl methacrylate) polycarbonate. Biomaterials; 32 (34), 8947-56

Altmann B, Ahrens R, Welle A, Dinglreiter H, Schneider M, Schober A. Microstructuring of multiwell plates for three-dimensional cell culture applications by ultrasonic embossing. Biomed Microdevices, 2012, 4(2):291-301

Altmann B, Welle A, Giselbrecht S, Truckenmüller R, Gottwald E. (2009). The famous versus the inconvenient – or the dawn and the rise of 3D-culture systems. World J Stem Cells; 1(1): 43-48

Altmann B, Giselbrecht S, Weibezahn KF, Welle A, Gottwald E. (2008). The three-dimensional cultivation of the hepatoma cell line HepG2 in a perfused chip system leads to a more differentiated phenotype of the cells compared to monolayer. Biomed. Mater.; 3 (3)

Gottwald E, Giselbrecht S, Augspurger C, Lahni B, Dambrowsky N, Truckenmuller R, Piotter V, Gietzelt T, Wendt O, Pfleging W, Welle A, Rolletschek A, Wobus AM, Weibezahn KF. A chip-based platform for the in vitro generation of tissues in three-dimensional organization. Lab Chip, 2007, 7 (6), 777-85

Dr. Brigitte Altmann, Ph.D.

Dept. of Oral, Maxillofacial, & Regional Plastic Surgery Freiburg University Medical Center

EDUCATION

Doctor of Philosophy (Biology), Heidelberg University and Institute for Biological Interfaces-1, Karlsruhe Institute of Technology, Germany

Postdoctoral
RESEARCH FELLOWSHIPS

Department of Prosthodontic Dentistry, Center for Dental Medicine, University of Freiburg, Germany

Institute for Biological Interfaces-1, Karlsruhe Institute of Technology, Germany